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KMID : 0368420080510010042
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Journal of Plant Biology
2008 Volume.51 No. 1 p.42 ~ p.47
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Molecular Cloning and Biochemical Analysis of Dihydroflavonol 4-Reductase (DFR) fromBrassica rapa ssp,pekinesis (Chinese Cabbage) using a Heterologous System
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Lee Won-Sook
You Jung-A
Chung Hee
Lee Youn-Hyung
Baek Nam-ln
Yoo Jong-Su
Park Young-Doo
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Abstract
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Dihydroflavonol 4-reductase (DFR) is part of an important step in the flavonoid biosynthesis pathway of anthocyanins. We isolated theDFR gene fromBrassica rapa ssp.pekinesis (Chinese cabbage), and found that biosynthesis of anthocyanins in tobacco could be modulated by introducing a heterologousDFR Bra gene. RT-PCR showed that transgenic plants had abnormal flower colors compared with the wild-type controls, a response that was correlated with the level ofDFR Bra transcription. HPLC analysis revealed that the isolated DFRBra cDNA mainly produced cyanidin as well as a small amount of pelargonidin in the anthocyanin biosynthesis pathway of those tobacco plants.
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KEYWORD
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anthocyanin, Chinese cabbage, DFR, flavonoid
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